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KMID : 0828520060100040285
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Journal of the Korean Geriatrics Society
2006 Volume.10 No. 4 p.285 ~ p.289
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Distribution Change of WD40 Repeat Protein 1 in Artificially Induced Senescent PC12 Cells
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Shin Dong-Hoon
Oh Chang-Seok
Lee Eun-Ju
Oh Seung-Ha
Lee Young-Soo
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Abstract
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Background: WDR1 is thought to be correlating with polymerization and depolymerization of actin protein. Though WDR1 was found to be within nucleus, in which actin could not be present by previous studies, the exact distribution pattern of WDR1 protein under various circumstances was not elucidated up to the present time. In this regard, we tried to see a change in the distribution of WDR1 protein within artificially induced senescent PC 12 pheochromocytoma cells for the first time.
Methods: PC12 pheochromocytoma cells (ATCC CRL-1721) were grown in the culture media including 1 M 3’-Azido-3’-deoxythymidine (AZT, Sigma-Aldrich, USA). The senescence of the cells was confirmed by senescence detection kit (Calbiochem, San Diego, CA). Immunocytochemical study by using WDR1 antibody was also performed in the cells treated with AZT during 0, 75 and 153 days.
Results: WDR1 protein was mainly observed within the cytoplasm of the cells not treated with AZT. However, the distribution of the same protein was changed into the nucleus after 153 day-AZT treatment.
Conclusion: The distribution of WDR1 protein was changed into nucleus in the artificially senescent PC12 cells.
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KEYWORD
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WD40 repeat protein 1, PC12, Senescence, 3-Azido-3-deoxythymidine
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